• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

FOXD3 Polyclonal antibody

FOXD3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27878-1-AP

Print datasheet

Synonyms

AIS1, forkhead box D3, Forkhead box protein D3, FOXD3, Genesis, HFH2

验证数据展示

  • WB analysis of HEK-293 using 27878-1-AP

    WB analysis of HEK-293 using 27878-1-AP

    WB result of FOXD3 antibody (27878-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXD3 transfected HEK-293 cells.

  • WB analysis of HEK-293 using 27878-1-AP

    WB analysis of HEK-293 using 27878-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27878-1-AP (FOXD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27878-1-AP targets FOXD3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen FOXD3 fusion protein Ag27260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box D3
Synonyms AIS1, forkhead box D3, Forkhead box protein D3, FOXD3, Genesis, HFH2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight57-60 kDa
GenBank Accession NumberNM_012183.2
Gene Symbol FOXD3
Gene ID (NCBI) 27022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXD3, also named as HFH2, belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. It also acts as a transcriptional activator. FOXD3 promotes development of neural crest cells from neural tube progenitors. It restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. It required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. FOXD3 has one isoform produced by alternative splicing with the MW of 47 kDa. It can be detected as 57-60 kDa in some literature(PMID: 31940493).

实验方案

Product Specific Protocols
WB protocol for FOXD3 antibody 27878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols