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验证数据展示

  • WB analysis of HEK-293 using 27878-1-AP

    WB analysis of HEK-293 using 27878-1-AP

    WB result of FOXD3 antibody (27878-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXD3 transfected HEK-293 cells.

  • WB analysis of HEK-293 using 27878-1-AP

    WB analysis of HEK-293 using 27878-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27878-1-AP (FOXD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

FOXD3 Polyclonal antibody

FOXD3 Polyclonal Antibody for WB, ELISA
Cat No. 27878-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

AIS1, forkhead box D3, Forkhead box protein D3, FOXD3, Genesis, HFH2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27878-1-AP targets FOXD3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 FOXD3 fusion protein Ag27260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box D3
别名 AIS1, forkhead box D3, Forkhead box protein D3, FOXD3, Genesis, HFH2
计算分子量 48 kDa
观测分子量57-60 kDa
GenBank蛋白编号NM_012183.2
基因名称 FOXD3
Gene ID (NCBI) 27022
RRIDAB_3086002
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXD3, also named as HFH2, belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. It also acts as a transcriptional activator. FOXD3 promotes development of neural crest cells from neural tube progenitors. It restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. It required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. FOXD3 has one isoform produced by alternative splicing with the MW of 47 kDa. It can be detected as 57-60 kDa in some literature(PMID: 31940493).

实验方案

Product Specific Protocols
WB protocol for FOXD3 antibody 27878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols