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验证数据展示

  • WB analysis of NIH/3T3 using 24835-1-AP

    WB analysis of NIH/3T3 using 24835-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FOXD4 Polyclonal antibody

FOXD4 Polyclonal Antibody for WB, ELISA
Cat No. 24835-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, Jurkat cells, RAW 264.7 cells, U-937 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24835-1-AP targets FOXD4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FOXD4 fusion protein Ag19226 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box D4
别名 FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha
计算分子量 439 aa, 47 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC136570
基因名称 FOXD4
Gene ID (NCBI) 2298
RRIDAB_2879750
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12950
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Forkhead box protein D4(FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.

实验方案

Product Specific Protocols
WB protocol for FOXD4 antibody 24835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols