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FOXD4 Polyclonal antibody

FOXD4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24835-1-AP

Print datasheet

Synonyms

FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha

验证数据展示

  • WB analysis of NIH/3T3 using 24835-1-AP

    WB analysis of NIH/3T3 using 24835-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24835-1-AP (FOXD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, Jurkat cells, RAW 264.7 cells, U-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24835-1-AP is FOXD4 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FOXD4 fusion protein Ag19226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box D4
Synonyms FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha
Calculated Molecular Weight 439 aa, 47 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC136570
Gene Symbol FOXD4
Gene ID (NCBI) 2298
RRIDAB_2879750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12950
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Forkhead box protein D4(FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.

实验方案

Product Specific Protocols
WB protocol for FOXD4 antibody 24835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols