验证数据展示
经过测试的应用
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
55363-1-AP targets FOXE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box E1 (thyroid transcription factor 2) |
| 别名 | FKHL15, Forkhead box protein E1, Forkhead box protein E2, FOXE1, FOXE2, HFKH4, HFKL5, HNF 3/fork head like protein 5, Thyroid transcription factor 2, TITF2, TTF 2, TTF2 |
| 计算分子量 | 38 kDa |
| GenBank蛋白编号 | NM_004473 |
| 基因名称 | FOXE1 |
| Gene ID (NCBI) | 2304 |
| RRID | AB_2935542 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00358 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXE1 is a thyroid-specific forkhead transcription factor essential for thyroid gland development, as well as for the maintenance of the thyroid differentiated state in adults. Mutations in FOXE1 are associated with congenital hypothyroidism and cleft palate with thyroid dysgenesis (PMID: 20453517, PMID: 25381600). FOXE1 is expressed in the developing thyroid and the anterior pituitary gland.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FOXE1 antibody 55363-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |