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验证数据展示

  • WB analysis of mouse liver using 20268-1-AP

    WB analysis of mouse liver using 20268-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20268-1-AP (FOXH1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

FOXH1-Specific Polyclonal antibody

FOXH1-Specific Polyclonal Antibody for WB, ELISA
Cat No. 20268-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

FAST 1, Fast 2, FAST1, FAST2, forkhead box H1, Forkhead box protein H1, FOXH1, hFAST 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20268-1-AP targets FOXH1-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box H1
别名 FAST 1, Fast 2, FAST1, FAST2, forkhead box H1, Forkhead box protein H1, FOXH1, hFAST 1
计算分子量 39 kDa
观测分子量 40-44 kDa
GenBank蛋白编号NM_003923
基因名称 FOXH1
Gene ID (NCBI) 8928
RRIDAB_10694676
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75593
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXH1, also named as FAST1 and FAST2, is a transcriptional activator. FOXH1 recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. It is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. It forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). The antibody is specific to FOXH1.

实验方案

Product Specific Protocols
WB protocol for FOXH1-Specific antibody 20268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols