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验证数据展示

  • WB analysis using 25903-1-AP

    WB analysis using 25903-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25903-1-AP (FOXJ1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 25903-1-AP

    IHC staining of mouse liver using 25903-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25903-1-AP (FOXJ1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FOXJ1 Polyclonal antibody

FOXJ1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25903-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HFH-4, HFH4, HFH 4, Forkhead-related protein FKHL13, FKHL13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25903-1-AP targets FOXJ1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FOXJ1 fusion protein Ag20827 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box J1
别名 HFH-4, HFH4, HFH 4, Forkhead-related protein FKHL13, FKHL13
计算分子量 45 kDa
观测分子量 50 kDa
GenBank蛋白编号BC046460
基因名称 FOXJ1
Gene ID (NCBI) 2302
RRIDAB_3669505
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92949
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXJ1 (Forkhead box protein J1) acts by activating the transcription of genes mediating motile cilia's assembly, such as CFAP157. It binds the DNA consensus sequences 5'-HWDTGTTTGTTTA-3' or 5'-KTTTGTTGTTKTW-3' (where H is not G, W is A or T, D is not C, and K is G or T), and activates the transcription of a variety of ciliary proteins in the developing brain and lung (PMID: 31630787).

实验方案

Product Specific Protocols
WB protocol for FOXJ1 antibody 25903-1-APDownload protocol
IHC protocol for FOXJ1 antibody 25903-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols