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FOXK1 Polyclonal antibody

FOXK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29338-1-AP

Print datasheet

Synonyms

MNF, FOXK1L

验证数据展示

  • WB analysis using 29338-1-AP

    WB analysis using 29338-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29338-1-AP

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29338-1-AP

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, MDA-MB-231 cells, MKN-45 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29338-1-AP targets FOXK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FOXK1 fusion protein Ag28820 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box K1
Synonyms MNF, FOXK1L
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberNM_001037165
Gene Symbol FOXK1
Gene ID (NCBI) 221937
RRIDAB_3669682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP85037
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).

实验方案

Product Specific Protocols
WB protocol for FOXK1 antibody 29338-1-APDownload protocol
IHC protocol for FOXK1 antibody 29338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols