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验证数据展示

  • WB analysis using 30660-1-AP

    WB analysis using 30660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30660-1-AP (FOXK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HAP1 using 30660-1-AP

    WB analysis of HAP1 using 30660-1-AP

    WB result of FOXK2 antibody (30660-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and FOXK2 knockout HAP1 cells.

  • IHC staining of mouse brain using 30660-1-AP

    IHC staining of mouse brain using 30660-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30660-1-AP (FOXK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXK2 antibody included
  • Featured Product
  • KD/KO验证

FOXK2 Polyclonal antibody

FOXK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30660-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

forkhead box K2, Forkhead box protein K2, ILF, ILF 1, ILF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HAP1 cells, HeLa cells, MCF-10A cells, MCF-7 cells, MDA-MB-231 cells, T-47D cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXK2 Ready-To-Use IHC Kit. FOXK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30660-1-AP targets FOXK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FOXK2 fusion protein Ag33625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box K2
别名 forkhead box K2, Forkhead box protein K2, ILF, ILF 1, ILF1
计算分子量69 kDa
观测分子量69-79 kDa
GenBank蛋白编号NM_004514.3
基因名称 FOXK2
Gene ID (NCBI) 3607
RRIDAB_3086382
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01167
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).

实验方案

Product Specific Protocols
WB protocol for FOXK2 antibody 30660-1-APDownload protocol
IHC protocol for FOXK2 antibody 30660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols