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Species Reactivity
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验证数据展示

  • WB analysis using 83735-4-RR (same clone as 83735-4-PBS)

    WB analysis using 83735-4-RR (same clone as 83735-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83735-4-RR (FOXK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 83735-4-RR (same clone as 83735-4-PBS)

    IHC staining of human placenta using 83735-4-RR (same clone as 83735-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-4-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXK2 antibody included
  • IF Staining of A431 using 83735-4-RR (same clone as 83735-4-PBS)

    IF Staining of A431 using 83735-4-RR (same clone as 83735-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 83735-4-RR (same clone as 83735-4-PBS)

    IF Staining of A431 using 83735-4-RR (same clone as 83735-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-4-RR, Clone: 240692A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 83735-4-RR (same clone as 83735-4-PBS)

    FC experiment of SH-SY5Y using 83735-4-RR (same clone as 83735-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 83735-4-RR (same clone as 83735-4-PBS)

    FC experiment of HEK-293T using 83735-4-RR (same clone as 83735-4-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug FOXK2 Recombinant antibody (83735-4-RR, Clone:240692A5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83735-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83735-4-RR

    Affinity and Kinetic Characterization of 83735-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83735-4-RR against Human FOXK2 were performed. The affinity constant is 0.119 nM.

FOXK2 Recombinant antibody, PBS Only

FOXK2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83735-4-PBS

产品说明书

CloneNo. 240692A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXK2 Ready-To-Use IHC Kit. FOXK2 primary antibody included.

产品信息

83735-4-PBS targets FOXK2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FOXK2 fusion protein Ag33625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box K2
别名 ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2
计算分子量69 kDa
观测分子量69-79 kDa
GenBank蛋白编号NM_004514.3
基因名称 FOXK2
Gene ID (NCBI) 3607
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ01167
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXK2 (forkhead box K2), also known as ILF and ILF1. FOXK2 is localized at nucleus and cytoplasm. It is also expected to be expressed in testis, brain and lymphoid and non-lymphoid cells (PMID: 1339390). The protein encoded by this gene contains a fork head DNA binding domain. This protein can bind to the purine-rich motifs of the HIV long terminal repeat (LTR), and to the similar purine-rich motif in the interleukin 2 (IL2) promoter. It may be involved in the regulation of viral and cellular promoter elements. And its molecular weight is 69 kDa. Anett Marais demonstrate that FOXK2 is phosphorylated in a cell cycle-dependent manner. This phosphorylation peaks during M phase and is mediated by CDK·cyclin complexes. FOXK2 exhibits a periodic rise in its phosphorylation levels during the cell cycle, with hyperphosphorylation occurring in mitotic cells (PMID: 20810654).