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FOXN3 Polyclonal antibody

FOXN3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25399-1-AP

Print datasheet

Synonyms

C14orf116, Checkpoint suppressor 1, CHES1, forkhead box N3, Forkhead box protein N3, FOXN3, PRO1635

验证数据展示

  • WB analysis using 25399-1-AP

    WB analysis using 25399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25399-1-AP (FOXN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 25399-1-AP

    IF Staining of U2OS using 25399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FOXN3 antibody (25399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse embryo tissue
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25399-1-AP targets FOXN3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FOXN3 fusion protein Ag21929 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box N3
Synonyms C14orf116, Checkpoint suppressor 1, CHES1, forkhead box N3, Forkhead box protein N3, FOXN3, PRO1635
Calculated Molecular Weight 490 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC007506
Gene Symbol FOXN3
Gene ID (NCBI) 1112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00409
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

As transcriptional regulators, the FOX protein family regulates the development of tissues, embryos as well as carbohydrate and lipid metabolism. Forkhead box N3 (FOXN3), also known as checkpoint suppressor 1 (CHES1), is initially identified from yeast and belongs to the FOX protein family as it contains a conserved FOX DNA binding domain in its N-terminal(PMID: 36450706). FOXN3 had been dysregulated in many types of malignancies including melanoma, osteosarcoma, hepatocellular carcinoma and breast cancer, and its expression level is strongly associated with progression and prognosis of patients(PMID: 31214487).

实验方案

Product Specific Protocols
WB protocol for FOXN3 antibody 25399-1-APDownload protocol
IF protocol for FOXN3 antibody 25399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols