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验证数据展示

  • WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

    WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

    WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

    WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FOXO1 Monoclonal antibody, PBS Only

FOXO1 Monoclonal Antibody for WB, ELISA
Cat No. 66457-1-PBS

产品说明书

CloneNo. 1D9A2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.

产品信息

66457-1-PBS targets FOXO1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FOXO1 fusion protein Ag13296 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 forkhead box O1
别名 FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A
计算分子量 655 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC021981
基因名称 FOXO1
Gene ID (NCBI) 2308
RRIDAB_2881826
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12778
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).