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  • Featured Product
  • KD/KO Validated

FOXO1 Monoclonal antibody, PBS Only

FOXO1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1D9A2

Cat No : 66457-1-PBS

Print datasheet

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

验证数据展示

  • WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

    WB analysis using 66457-1-Ig (same clone as 66457-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66457-1-Ig (FOXO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

    WB analysis of HEK-293 using 66457-1-Ig (same clone as 66457-1-PBS)

    WB result of FOXO1 antibody (66457-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66457-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.

产品信息

66457-1-PBS targets FOXO1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA Application Description
Tested Reactivity human
Immunogen FOXO1 fusion protein Ag13296 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name forkhead box O1
Synonyms FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A
Calculated Molecular Weight 655 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC021981
Gene Symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12778
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).