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FOXO4 Polyclonal antibody

FOXO4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19121-1-AP

Print datasheet

Synonyms

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

验证数据展示

  • WB analysis of human heart using 19121-1-AP

    WB analysis of human heart using 19121-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19121-1-AP (FOXO4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19121-1-AP

    IF Staining of HepG2 using 19121-1-AP

    Immunofluorescent analysis of HepG2 cells, using FOXO4 antibody 19121-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman heart tissue, mouse heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FOXO4 Ready-To-Use IHC Kit. FOXO4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19121-1-AP targets FOXO4 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box O4
Synonyms AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberNM_005938
Gene Symbol FOXO4
Gene ID (NCBI) 4303
RRIDAB_2106957
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP98177
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the ins signaling pathway. It binds to ins-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

实验方案

Product Specific Protocols
WB protocol for FOXO4 antibody 19121-1-APDownload protocol
IF protocol for FOXO4 antibody 19121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Neurosci Lett

Expression of FOXO transcription factors in the brain following traumatic brain injury.

Authors - Xi-Lin Liu
View Article