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FOXO4 Polyclonal antibody

FOXO4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21535-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, ELISA and More (2)

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-12 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXO4 Ready-To-Use IHC Kit. FOXO4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21535-1-AP targets FOXO4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine, bovine
免疫原 FOXO4 fusion protein Ag16169 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box O4
别名 AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7
计算分子量 505 aa, 54 kDa
观测分子量 65 kDa
GenBank蛋白编号BC106761
基因名称 FOXO4
Gene ID (NCBI) 4303
RRIDAB_10732960
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP98177
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

实验方案

Product Specific Protocols
WB protocol for FOXO4 antibody 21535-1-APDownload protocol
IHC protocol for FOXO4 antibody 21535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB

Circ Res

Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.

Authors - Gaelle Auguste
humanWB

J Clin Invest

Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.

Authors - Xu Xiaojie X
  • KD Validated
mouse,ratWB,IHC

Nat Commun

Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.

Authors - Wei Xia
ratWB

Redox Biol

Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.

Authors - Han Li
humanIF

Mater Today Bio

Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence

Authors - Jiamei Han
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
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