验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, Neuro-2a cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
| Positive IP detected in | mouse spleen tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20529-1-AP targets FOXP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | FOXP2 fusion protein Ag14391 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | forkhead box P2 |
| Synonyms | CAG repeat protein 44, CAGH44, forkhead box P2, Forkhead box protein P2, FOXP2, SPCH1, TNRC10 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC018016 |
| Gene Symbol | FOXP2 |
| Gene ID (NCBI) | 93986 |
| RRID | AB_10695756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15409 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXP2, Forkhead box protein P2, is associated with speech and language development (PMID: 24765219). FOXP2 is expressed in the brain, lungs, heart and gut. In the brain, it is widely expressed in sensory, limbic and motor structures(PMID: 10880297, PMID:15685218). Mutations in FOXP2 caused the severe speech and language disorder developmental verbal dyspraxia (PMID: 15877281). FOXP2 was detected 2 isoforms of 70 kDa and 80-82 kDa (PMID: 15056696, PMID: 29360038, PMID: 17999357).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| IP protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways. | ||
J Cancer Low expression of FOXP2 predicts poor survival and targets caspase-1 to inhibit cell pyroptosis in colorectal cancer.
| ||
BMC Biol GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion | ||
Commun Biol FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior |