FOXP3 Polyclonal antibody
FOXP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22228-1-AP targets FOXP3 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IP, CoIP, ChIP, ELISA |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | FOXP3 fusion protein Ag17571 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | forkhead box P3 |
Synonyms | Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER |
Calculated Molecular Weight | 431 aa, 47 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC113401 |
Gene Symbol | FOXP3 |
Gene ID (NCBI) | 50943 |
RRID | AB_11182376 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BZS1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for FOXP3 antibody 22228-1-AP | Download protocol |
IHC protocol for FOXP3 antibody 22228-1-AP | Download protocol |
IP protocol for FOXP3 antibody 22228-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Pharm Sin B Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy | ||
Cancer Res Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma. | ||
Biomaterials LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy. | ||
Clin Transl Med MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis. | ||
Biomed Pharmacother Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis |