FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22228-1-AP

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Synonyms

Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22228-1-AP targets FOXP3 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, ChIP, ELISA
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Immunogen FOXP3 fusion protein Ag17571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box P3
Synonyms Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC113401
Gene Symbol FOXP3
Gene ID (NCBI) 50943
RRIDAB_11182376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZS1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IHC protocol for FOXP3 antibody 22228-1-APDownload protocol
IP protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Acta Pharm Sin B

Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy

Authors - Hao Ren
mouse

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li
mouse

Biomaterials

LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.

Authors - Feng Liu
humanWB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Autoimmun

NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.

Authors - Mengxiao Xie
humanWB

Biomed Pharmacother

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Authors - Qiuyan Guo