FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 22228-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (3)

应用

WB, IHC, IF-P, ELISA and More (4)

Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A375 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy FOXP3 Ready-To-Use IHC Kit. FOXP3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22228-1-AP targets FOXP3 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, ELISA
经测试反应性 human
文献引用反应性human, mouse, rat, pig
免疫原 FOXP3 fusion protein Ag17571 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box P3
别名 Forkhead box protein P3, C-terminally processed, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, forkhead box P3, DIETER
计算分子量 431 aa, 47 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC113401
基因名称 FOXP3
Gene ID (NCBI) 50943
RRIDAB_11182376
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZS1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

实验方案

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IHC protocol for FOXP3 antibody 22228-1-APDownload protocol
IF protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Acta Pharm Sin B

Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy

Authors - Hao Ren
mouse

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li
mouse

Biomaterials

LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.

Authors - Feng Liu
humanWB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Autoimmun

NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.

Authors - Mengxiao Xie
humanWB

Biomed Pharmacother

Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis

Authors - Qiuyan Guo
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