验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human thyroid cancer tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
| Positive FC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy FOXP4 Ready-To-Use IHC Kit. FOXP4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16772-1-AP targets FOXP4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FOXP4 fusion protein Ag10047 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box P4 |
| 别名 | FKHLA, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA |
| 计算分子量 | 678 aa, 73 kDa |
| 观测分子量 | 73 kDa |
| GenBank蛋白编号 | BC052803 |
| 基因名称 | FOXP4 |
| Gene ID (NCBI) | 116113 |
| RRID | AB_2262825 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVH2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IHC protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IF protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IP protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Lipid Res The Forkhead Box Transcription Factor FoxP4 Regulates Thermogenic Programs in Adipocytes.
| ||
Mol Med Rep Downregulation of microRNA‑423‑5p suppresses TGF‑β1‑induced EMT by targeting FOXP4 in airway fibrosis. | ||
Heliyon Transcription factor FOXP4 inversely governs tumor suppressor genes and contributes to thyroid cancer progression
| ||
Cancer Sci FOXP4 inhibits squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway | ||
Cancer Med Androgen promotes squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway |
