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FOXP4 Polyclonal antibody

FOXP4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29311-1-AP

Print datasheet

Synonyms

FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA

验证数据展示

  • WB analysis using 29311-1-AP

    WB analysis using 29311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29311-1-AP (FOXP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 29311-1-AP

    IHC staining of mouse ovary using 29311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP4 antibody included

经过测试的应用

Positive WB detected inCaco-2 cells, PC-3 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXP4 Ready-To-Use IHC Kit. FOXP4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29311-1-AP targets FOXP4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen FOXP4 fusion protein Ag29953 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box P4
Synonyms FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA
Calculated Molecular Weight 678 aa, 73 kDa
Observed Molecular Weight73 kDa
GenBank Accession NumberBC052803
Gene Symbol FOXP4
Gene ID (NCBI) 116113
RRIDAB_2918279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVH2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.

实验方案

Product Specific Protocols
WB protocol for FOXP4 antibody 29311-1-APDownload protocol
IHC protocol for FOXP4 antibody 29311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols