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验证数据展示

  • WB analysis using 29311-1-AP

    WB analysis using 29311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29311-1-AP (FOXP4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 29311-1-AP

    IHC staining of mouse ovary using 29311-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29311-1-AP (FOXP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXP4 antibody included

FOXP4 Polyclonal antibody

FOXP4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29311-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, PC-3 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FOXP4 Ready-To-Use IHC Kit. FOXP4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29311-1-AP targets FOXP4 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 FOXP4 fusion protein Ag29953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box P4
别名 FKHLA, FLJ40908, FLJ44184, forkhead box P4, Forkhead box protein P4, FOXP4, hFKHLA
计算分子量 678 aa, 73 kDa
观测分子量73 kDa
GenBank蛋白编号BC052803
基因名称 FOXP4
Gene ID (NCBI) 116113
RRIDAB_2918279
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVH2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP4, also named as FKHLA, belongs to the FOX family. FOXP4 has been implicated in diverse biological processes in tumor initiation and progression. It has been demonstrated that FOXP4 is significantly upregulated in breast cancer, hepatocellular carcinoma and oral squamous cell carcinoma (PMID: 34590150). FOXP4 has 3 isoforms with the molecular mass of 72-73 kDa.

实验方案

Product Specific Protocols
WB protocol for FOXP4 antibody 29311-1-APDownload protocol
IHC protocol for FOXP4 antibody 29311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols