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FPGT Polyclonal antibody

FPGT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24937-1-AP

Print datasheet

Synonyms

FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP

验证数据展示

  • WB analysis of mouse colon using 24937-1-AP

    WB analysis of mouse colon using 24937-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 24937-1-AP (FPGT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24937-1-AP

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 24937-1-AP

    IHC staining of human colon using 24937-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24937-1-AP (FPGT Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24937-1-AP targets FPGT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FPGT fusion protein Ag20655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucose-1-phosphate guanylyltransferase
Synonyms FPGT, GDP L fucose diphosphorylase, GDP L fucose pyrophosphorylase, GFPP
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC032308
Gene Symbol FPGT
Gene ID (NCBI) 8790
RRIDAB_2879808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FPGT(Fucose-1-phosphate guanylyltransferase) is also named as GFPP. This protein catalyzes the formation of GDP-L-fucose from GTP and L-fucose-1-phosphate. It also functions as a salvage pathway to reutilize L-fucose arising from the turnover of glycoproteins and glycolipids. FPGT has 6 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FPGT antibody 24937-1-APDownload protocol
IHC protocol for FPGT antibody 24937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols