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验证数据展示

  • WB analysis of HEK-293 using 30989-1-AP

    WB analysis of HEK-293 using 30989-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 30989-1-AP (FPR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30989-1-AP

    IHC staining of mouse brain using 30989-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30989-1-AP (FPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FPR2 Polyclonal antibody

FPR2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30989-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

FMLP-R-I, FMLP-related receptor I, FMLP related receptor I, FMLP R II, FMLP R I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30989-1-AP targets FPR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FPR2 fusion protein Ag34304 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 formyl peptide receptor 2
别名 FMLP-R-I, FMLP-related receptor I, FMLP related receptor I, FMLP R II, FMLP R I
计算分子量 351 aa, 39 kDa
观测分子量42 kDa
GenBank蛋白编号BC029125
基因名称 FPR2
Gene ID (NCBI) 2358
RRIDAB_3669809
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP25090
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The formyl peptide receptors (FPRs) are a group of G protein-coupled chemoattractant receptors that play important roles in host defense and inflammation (PMID: 28335409). In humans, three different isoforms are expressed (FPR1, FPR2, and FPR3). FPR2, also known as FPRL1 or ALX, is expressed by many cell types including monocytes, neutrophils, macrophages, astrocytoma cells, and epithelial cells. FPR2 conveys the biological functions of a variety of ligands, including N-formyl-methionyl peptides, the pro-resolution mediators annexin A1 (AnxA1) and lipoxin A4, as well as the activating and proinflammatory protein serum amyloid A (PMID: 22610094).

实验方案

Product Specific Protocols
WB protocol for FPR2 antibody 30989-1-APDownload protocol
IHC protocol for FPR2 antibody 30989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols