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验证数据展示

  • WB analysis of HEK-293 using 30989-1-AP

    WB analysis of HEK-293 using 30989-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 30989-1-AP (FPR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30989-1-AP

    IHC staining of mouse brain using 30989-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30989-1-AP (FPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FPR2 Polyclonal antibody

FPR2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30989-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

FMLP-R-I, FMLP-related receptor I, FMLP related receptor I, FMLP R II, FMLP R I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30989-1-AP targets FPR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FPR2 fusion protein Ag34304 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name formyl peptide receptor 2
Synonyms FMLP-R-I, FMLP-related receptor I, FMLP related receptor I, FMLP R II, FMLP R I
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC029125
Gene Symbol FPR2
Gene ID (NCBI) 2358
RRIDAB_3669809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP25090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The formyl peptide receptors (FPRs) are a group of G protein-coupled chemoattractant receptors that play important roles in host defense and inflammation (PMID: 28335409). In humans, three different isoforms are expressed (FPR1, FPR2, and FPR3). FPR2, also known as FPRL1 or ALX, is expressed by many cell types including monocytes, neutrophils, macrophages, astrocytoma cells, and epithelial cells. FPR2 conveys the biological functions of a variety of ligands, including N-formyl-methionyl peptides, the pro-resolution mediators annexin A1 (AnxA1) and lipoxin A4, as well as the activating and proinflammatory protein serum amyloid A (PMID: 22610094).

实验方案

Product Specific Protocols
WB protocol for FPR2 antibody 30989-1-APDownload protocol
IHC protocol for FPR2 antibody 30989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols