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FRK Polyclonal antibody

FRK Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16197-1-AP

Print datasheet

Synonyms

Protein-tyrosine kinase 5, Protein tyrosine kinase 5, GTK, FYN-related kinase, fyn related kinase

验证数据展示

  • WB analysis using 16197-1-AP

    WB analysis using 16197-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16197-1-AP (FRK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16197-1-AP

    IF Staining of HepG2 using 16197-1-AP

    Immunofluorescent analysis of HepG2 cells, using FRK antibody 16197-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16197-1-AP targets FRK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FRK fusion protein Ag10372 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fyn-related kinase
Synonyms Protein-tyrosine kinase 5, Protein tyrosine kinase 5, GTK, FYN-related kinase, fyn related kinase
Calculated Molecular Weight 505 aa, 56 kDa
Observed Molecular Weight 54-57 kDa
GenBank Accession NumberBC012916
Gene Symbol FRK
Gene ID (NCBI) 2444
RRIDAB_10639525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named PTK5 (protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).

实验方案

Product Specific Protocols
WB protocol for FRK antibody 16197-1-APDownload protocol
IF protocol for FRK antibody 16197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cancer Lett

FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia

Authors - Yuxin Tan
View Article