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验证数据展示

  • WB analysis of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    WB analysis of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-Ig (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FRS2 antibody included
  • IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    IHC staining of human prostate cancer using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66263-1-Ig (FRS2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FRS2 antibody included
  • IF Staining of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    IF Staining of MCF-7 using 66263-1-Ig (same clone as 66263-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FRS2 antibody (66263-1-Ig, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66263-1-PBS in a different storage buffer formulation.

FRS2 Monoclonal antibody, PBS Only

FRS2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66263-1-PBS

产品说明书

CloneNo. 4E10H12

宿主/亚型

Mouse / IgG3

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FRS2 Ready-To-Use IHC Kit. FRS2 primary antibody included.

产品信息

66263-1-PBS targets FRS2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FRS2 fusion protein Ag19299 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 fibroblast growth factor receptor substrate 2
别名 SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT, FGFR substrate 2
计算分子量 60 kDa
观测分子量 68 kDa
GenBank蛋白编号BC021562
基因名称 FRS2
Gene ID (NCBI) 10818
RRIDAB_2881650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8WU20
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.