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验证数据展示

  • WB analysis of mouse heart using 25609-1-AP

    WB analysis of mouse heart using 25609-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 25609-1-AP

    WB analysis using 25609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25609-1-AP (FSD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with .

  • IHC staining of mouse heart using 25609-1-AP

    IHC staining of mouse heart using 25609-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25609-1-AP (FSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .

FSD2 Polyclonal antibody

FSD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25609-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

FSD2, RP11 127F21, SPRYD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25609-1-AP targets FSD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FSD2 fusion protein Ag22229 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibronectin type III and SPRY domain containing 2
别名 FSD2, RP11 127F21, SPRYD1
计算分子量 749 aa, 85 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC130569
基因名称 FSD2
Gene ID (NCBI) 123722
RRIDAB_2880159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA1L4K1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FSD2 antibody 25609-1-APDownload protocol
IHC protocol for FSD2 antibody 25609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols