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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68111-1-Ig (same clone as 68111-1-PBS)

    WB analysis using 68111-1-Ig (same clone as 68111-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68111-1-Ig (FTO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

    IHC staining of mouse brain using 68111-1-Ig (same clone as 68111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68111-1-Ig (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FTO antibody included
  • FC experiment of SH-SY5Y using 68111-1-Ig (same clone as 68111-1-PBS)

    FC experiment of SH-SY5Y using 68111-1-Ig (same clone as 68111-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (68111-1-Ig, Clone:1E8B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68111-1-PBS in a different storage buffer formulation.

FTO Monoclonal antibody, PBS Only

FTO Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 68111-1-PBS

产品说明书

CloneNo. 1E8B1

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, pig

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

1E8B1, KIAA1752

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FTO Ready-To-Use IHC Kit. FTO primary antibody included.

产品信息

68111-1-PBS targets FTO in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, pig
免疫原 FTO fusion protein Ag26095 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fat mass and obesity associated
别名 1E8B1, KIAA1752
计算分子量 58 kDa
观测分子量58 kDa
GenBank蛋白编号NM_001080432
基因名称 FTO
Gene ID (NCBI) 79068
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9C0B1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.