验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, Jurkat cells, K-562 cells, mouse brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| ChIP | See 1 publications below |
产品信息
24864-1-AP targets FUBP1 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | FUBP1 fusion protein Ag13149 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | far upstream element (FUSE) binding protein 1 |
| 别名 | FUSE-binding protein 1, Far upstream element-binding protein 1 |
| 计算分子量 | 653 aa, 69 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC017247 |
| 基因名称 | FUBP1 |
| Gene ID (NCBI) | 8880 |
| RRID | AB_2879762 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AE4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| IF protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| IP protocol for FUBP1 antibody 24864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Poly(U) binding splicing factor 60 promotes renal cell carcinoma growth by transcriptionally upregulating telomerase reverse transcriptase. | ||
Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. | ||
Anticancer Res Comparative Proteomic Profiling of Cisplatin-resistant Nasopharyngeal Carcinoma Cell Lines: Novel Biomarkers for Improving Chemotherapy of NPC. | ||
J Adv Res LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1 | ||
MedComm (2020) Far upstream element-binding protein 1 confers lobaplatin resistance by transcriptionally activating PTGES and facilitating the arachidonic acid metabolic pathway in osteosarcoma | ||
J Immunother Cancer The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development
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