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FUCA1 Monoclonal antibody, PBS Only

FUCA1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat no : 67251-1-PBS

Print datasheet

Synonyms

FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1

验证数据展示

  • WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    IF Staining of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FUCA1 Ready-To-Use IHC Kit. FUCA1 primary antibody included.

产品信息

67251-1-PBS targets FUCA1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen FUCA1 fusion protein Ag9638 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fucosidase, alpha-L- 1, tissue
Synonyms FUCA, EC:3.2.1.51, Alpha-L-fucoside fucohydrolase 1, Alpha-L-fucosidase I, Alpha-L-fucosidase 1
Calculated Molecular Weight 461 aa, 53 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC017338
Gene Symbol FUCA1
Gene ID (NCBI) 2517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04066
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).