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FUCA1 Monoclonal antibody, PBS Only

FUCA1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G5C5

Cat No : 67251-1-PBS

Print datasheet

Synonyms

Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase

验证数据展示

  • WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    WB analysis of HepG2 using 67251-1-Ig (same clone as 67251-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67251-1-Ig (FUCA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IHC staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67251-1-Ig (FUCA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FUCA1 antibody included
  • IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    IF Staining of human liver cancer using 67251-1-Ig (same clone as 67251-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FUCA1 antibody (67251-1-Ig, Clone: 1G5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67251-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FUCA1 Ready-To-Use IHC Kit. FUCA1 primary antibody included.

产品信息

67251-1-PBS targets FUCA1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse
Immunogen FUCA1 fusion protein Ag9638 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name fucosidase, alpha-L- 1, tissue
Synonyms Alpha L fucosidase 1, Alpha L fucosidase I, FUCA, FUCA1, fucosidase, alpha L 1, tissue, Tissue alpha L fucosidase
Calculated Molecular Weight 461 aa, 53 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC017338
Gene Symbol FUCA1
Gene ID (NCBI) 2517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04066
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

FUCA1(a-l-Fucosidase) is a lysosomal glycosidase which is ubiquitous in eukaryotic cells. This enzyme is usually found as a soluble component of the lysosome and functions as an acid hydrolase in the degradation of numerous and diverse fucoglycoconjugates.The mammalian a-l-fucosidases are relatively large, multisubunit glycoproteins which exist in multiple molecular isoforms.This enzyme may have a role in sperm-egg interactions in the reproductive tract of the female rat. There is a precursor 54 kDa form of rat sperm fucosidase which is processed to a mature 52 kDa form of the enzyme(PMID:8836125). This protein from rat epididymis is a tetramer of 210-220 kDa, made up by two pairs of subunits of 47 and 60 kDa, and the one from human seminal plasma exists in different oligomeric forms, that is, as a dimer, as a tetramer, and as a hexamer made up of a single subunit of 56-57 kDa(PMID:16736526).