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FUCA2 Polyclonal antibody

FUCA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15157-1-AP

Print datasheet

Synonyms

Alpha L fucosidase 2, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260

验证数据展示

  • IP experiment of mouse heart using 15157-1-AP

    IP experiment of mouse heart using 15157-1-AP

    IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug.

  • WB analysis of human heart using 15157-1-AP

    WB analysis of human heart using 15157-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15157-1-AP

    IHC staining of human liver using 15157-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 15157-1-AP

    IHC staining of human liver using 15157-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman heart tissue, mouse stomach tissue, rat stomach tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15157-1-AP targets FUCA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FUCA2 fusion protein Ag7250 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucosidase, alpha-L- 2, plasma
Synonyms Alpha L fucosidase 2, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC003060
Gene Symbol FUCA2
Gene ID (NCBI) 2519
RRIDAB_2104956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTY2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

实验方案

Product Specific Protocols
WB protocol for FUCA2 antibody 15157-1-APDownload protocol
IHC protocol for FUCA2 antibody 15157-1-APDownload protocol
IP protocol for FUCA2 antibody 15157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis

Authors - Xiaoqiong Wei
View Article