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FUCA2 Polyclonal antibody, PBS Only

FUCA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30080-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 30080-1-AP (same clone as 30080-1-PBS)

    WB analysis using 30080-1-AP (same clone as 30080-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 30080-1-AP (same clone as 30080-1-PBS)

    IF Staining of HepG2 using 30080-1-AP (same clone as 30080-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30080-1-AP (same clone as 30080-1-PBS)

    IHC staining of human stomach cancer using 30080-1-AP (same clone as 30080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30080-1-AP (FUCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30080-1-PBS in a different storage buffer formulation.

产品信息

30080-1-PBS targets FUCA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen FUCA2 fusion protein Ag32467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucosidase, alpha-L- 2, plasma
Synonyms
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC003060
Gene Symbol FUCA2
Gene ID (NCBI) 2519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTY2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.