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FUK Polyclonal antibody

FUK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13541-1-AP

Print datasheet

Synonyms

1110046B12Rik, fucokinase, FUK, L fucose kinase

验证数据展示

  • WB analysis using 13541-1-AP

    WB analysis using 13541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 13541-1-AP

    IHC staining of mouse kidney using 13541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13541-1-AP (FUK Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, BxPC-3 cells, mouse thymus tissue, mouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13541-1-AP targets FUK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen FUK fusion protein Ag4481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucokinase
Synonyms 1110046B12Rik, fucokinase, FUK, L fucose kinase
Calculated Molecular Weight 1084 aa, 118 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC032542
Gene Symbol FUK
Gene ID (NCBI) 197258
RRIDAB_2104961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0W3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUK, also named FCSK, belongs to the GHMP kinase family. FUK encodes fucokinase, the only enzyme capable of converting L-fucose to fucose-1-phosphate, which will ultimately be used for synthesizing GDP-fucose, the donor substrate for all fucosyltransferases (PMID: 30503518).

实验方案

Product Specific Protocols
WB protocol for FUK antibody 13541-1-APDownload protocol
IHC protocol for FUK antibody 13541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols