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验证数据展示

  • WB analysis using 13541-1-AP

    WB analysis using 13541-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13541-1-AP (FUK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 13541-1-AP

    IHC staining of mouse kidney using 13541-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13541-1-AP (FUK Antibody) at dilution of 1:50 (under 40x lens).

FUK Polyclonal antibody

FUK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13541-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

1110046B12Rik, fucokinase, FUK, L fucose kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, BxPC-3 cells, mouse thymus tissue, mouse kidney tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13541-1-AP targets FUK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FUK fusion protein Ag4481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucokinase
别名 1110046B12Rik, fucokinase, FUK, L fucose kinase
计算分子量 1084 aa, 118 kDa
观测分子量 107 kDa
GenBank蛋白编号BC032542
基因名称 FUK
Gene ID (NCBI) 197258
RRIDAB_2104961
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0W3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUK, also named FCSK, belongs to the GHMP kinase family. FUK encodes fucokinase, the only enzyme capable of converting L-fucose to fucose-1-phosphate, which will ultimately be used for synthesizing GDP-fucose, the donor substrate for all fucosyltransferases (PMID: 30503518).

实验方案

Product Specific Protocols
WB protocol for FUK antibody 13541-1-APDownload protocol
IHC protocol for FUK antibody 13541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols