验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, RAW 264, .7 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue, C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
28519-1-AP targets FUNDC1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey, goat |
| 免疫原 | FUNDC1 fusion protein Ag29358 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FUN14 domain containing 1 |
| 别名 | FUN14 domain containing 1, FUNDC1 |
| 计算分子量 | 155 aa, 17 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC042813 |
| 基因名称 | FUNDC1 |
| Gene ID (NCBI) | 139341 |
| RRID | AB_2935469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVP5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FUNDC1 is a novel mitochondrial-associated membrane (MAM) protein, enriched at the MAM by interacting with the ER resident protein CANX (calnexin) under hypoxia. As mitophagy proceeds, it dissociates from CANX and preferably recruits DNM1L/DRP1 to drive mitochondrial fission in response to hypoxic stress.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUNDC1 antibody 28519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Sci (Lond) Loss of FoxO1 activates an alternate mechanism of mitochondrial quality control for healthy adipose browning
| ||
FASEB J Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system | ||
Mech Ageing Dev Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice | ||
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1 | ||
J Exp Clin Cancer Res Synthetic lethality of combined ULK1 defection and p53 restoration induce pyroptosis by directly upregulating GSDME transcription and cleavage activation through ROS/NLRP3 signaling | ||
PLoS Pathog Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation |
