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CoraLite® Plus 647-conjugated FUS/TLS Monoclonal antibody

FUS/TLS Monoclonal Antibody for IF/ICC
Cat No. CL647-60160

产品说明书

CloneNo. 3A10B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FUS/TLS Ready-To-Use IHC Kit. FUS/TLS primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-60160 targets FUS/TLS in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原 FUS/TLS fusion protein Ag2150 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fusion (involved in t(12;16) in malignant liposarcoma)
别名 FUS, ALS6, ALS, 75 kDa DNA-pairing protein, 75 kDa DNA pairing protein
计算分子量 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC026062
基因名称 FUS/TLS
Gene ID (NCBI) 2521
RRIDAB_2934980
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35637
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 FUS/TLS antibody CL647-60160Download protocol
Standard Protocols
Click here to view our Standard Protocols
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