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FUT10 Polyclonal antibody

FUT10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18660-1-AP

Print datasheet

Synonyms

Fuc TX, Fucosyltransferase 10, Fucosyltransferase X, FucT X, FUT10

验证数据展示

  • WB analysis of HeLa using 18660-1-AP

    WB analysis of HeLa using 18660-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18660-1-AP (FUT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18660-1-AP

    IHC staining of human testis using 18660-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18660-1-AP

    IHC staining of human testis using 18660-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18660-1-AP (FUT10 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 18660-1-AP is FUT10 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen FUT10 fusion protein Ag13415 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucosyltransferase 10 (alpha (1,3) fucosyltransferase)
Synonyms Fuc TX, Fucosyltransferase 10, Fucosyltransferase X, FucT X, FUT10
Calculated Molecular Weight 521 aa, 61 kDa
Observed Molecular Weight 41-48 kDa
GenBank Accession NumberBC063462
Gene Symbol FUT10
Gene ID (NCBI) 84750
RRIDAB_10641997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P4F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FUT10 antibody 18660-1-APDownload protocol
IHC protocol for FUT10 antibody 18660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Elife

The role of the NMD factor UPF3B in olfactory sensory neurons.

Authors - Kun Tan
View Article
humanWB

Arthritis Res Ther

Elevation of α-1,3 fucosylation promotes the binding ability of TNFR1 to TNF-α and contributes to osteoarthritic cartilage destruction and apoptosis.

Authors - Hanjie Yu
View Article