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  • Featured Product
  • KD/KO Validated

FUT7 Polyclonal antibody

FUT7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18197-1-AP

Print datasheet

Synonyms

Galactoside 3-L-fucosyltransferase, FUT 7, FucT-VII, Fuc-TVII, EC:2.4.1.-

验证数据展示

  • IHC staining of human breast cancer using 18197-1-AP

    IHC staining of human breast cancer using 18197-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 18197-1-AP

    IHC staining of human breast cancer using 18197-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18197-1-AP (FUT7 Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of HeLa using 18197-1-AP

    WB analysis of HeLa using 18197-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18197-1-AP (FUT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 18197-1-AP

    IF Staining of HeLa using 18197-1-AP

    Immunofluorescent analysis of HeLa cells using 18197-1-AP (FUT7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18197-1-AP targets FUT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FUT7 fusion protein Ag12475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucosyltransferase 7 (alpha (1,3) fucosyltransferase)
Synonyms Galactoside 3-L-fucosyltransferase, FUT 7, FucT-VII, Fuc-TVII, EC:2.4.1.-
Calculated Molecular Weight 342 aa, 39 kDa
Observed Molecular Weight 39-50 kDa
GenBank Accession NumberBC074746
Gene Symbol FUT7
Gene ID (NCBI) 2529
RRIDAB_2878516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ11130
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FUT7 antibody 18197-1-APDownload protocol
IHC protocol for FUT7 antibody 18197-1-APDownload protocol
IF protocol for FUT7 antibody 18197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC, WB

J Inflamm Res

FUT7 Promotes the Epithelial-Mesenchymal Transition and Immune Infiltration in Bladder Urothelial Carcinoma.

Authors - Mulin Liu
View Article
humanWB,IHC

Exp Cell Res

FUT7 promotes the malignant transformation of follicular thyroid carcinoma through α1,3-Fucosylation of EGF receptor.

Authors - Huamin Qin
  • KD Validated
View Article
humanWB

Evid Based Complement Alternat Med

Fut7 Promotes Adhesion and Invasion of Acute Lymphoblastic Leukemia Cells through the Integrin/Fak/Akt Pathway.

Authors - Fei He
View Article
humanWB,IHC,IF

Int J Mol Med

miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α

Authors - Xiaodan Wang
  • KD Validated
View Article