验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18197-1-AP targets FUT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FUT7 fusion protein Ag12475 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fucosyltransferase 7 (alpha (1,3) fucosyltransferase) |
| 别名 | EC:2.4.1.-, Fuc-TVII, FucT-VII, FUT 7, Galactoside 3-L-fucosyltransferase |
| 计算分子量 | 342 aa, 39 kDa |
| 观测分子量 | 39-50 kDa |
| GenBank蛋白编号 | BC074746 |
| 基因名称 | FUT7 |
| Gene ID (NCBI) | 2529 |
| RRID | AB_2878516 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q11130 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FUT7 antibody 18197-1-AP | Download protocol |
| IHC protocol for FUT7 antibody 18197-1-AP | Download protocol |
| IF protocol for FUT7 antibody 18197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Med miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α
| ||
J Inflamm Res FUT7 Promotes the Epithelial-Mesenchymal Transition and Immune Infiltration in Bladder Urothelial Carcinoma. | ||
Exp Cell Res FUT7 promotes the malignant transformation of follicular thyroid carcinoma through α1,3-Fucosylation of EGF receptor.
| ||
Evid Based Complement Alternat Med Fut7 Promotes Adhesion and Invasion of Acute Lymphoblastic Leukemia Cells through the Integrin/Fak/Akt Pathway. |
