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验证数据展示

  • WB analysis using 29430-1-AP

    WB analysis using 29430-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29430-1-AP (FUT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29430-1-AP

    IHC staining of human lung cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 29430-1-AP

    IHC staining of human breast cancer using 29430-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29430-1-AP (FUT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FUT8 Polyclonal antibody

FUT8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29430-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29430-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen FUT8 fusion protein Ag30784 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Synonyms Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8
Calculated Molecular Weight 66 kDa
Observed Molecular Weight60-66 kDa
GenBank Accession NumberBC025385
Gene Symbol FUT8
Gene ID (NCBI) 2530
RRIDAB_2918305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FUT8 antibody 29430-1-APDownload protocol
IHC protocol for FUT8 antibody 29430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols