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  • Featured Product
  • KD/KO Validated

FUT8 Monoclonal antibody, PBS Only

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7H2

Cat No : 66118-1-PBS

Print datasheet

Synonyms

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

验证数据展示

  • WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

产品信息

66118-1-PBS targets FUT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen FUT8 fusion protein Ag18769 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Synonyms Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC025385
Gene Symbol FUT8
Gene ID (NCBI) 2530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BYC5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).