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验证数据展示

  • WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MDA-MB-231 using 66118-1-Ig (same clone as 66118-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66118-1-Ig (FUT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB analysis of MCF-7 using 66118-1-Ig (same clone as 66118-1-PBS)

    WB result of FUT8 antibody (66118-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells. This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    IHC staining of human colon cancer using 66118-1-Ig (same clone as 66118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66118-1-Ig (FUT8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66118-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FUT8 Monoclonal antibody, PBS Only

FUT8 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66118-1-PBS

产品说明书

CloneNo. 2F7H2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66118-1-PBS targets FUT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 FUT8 fusion protein Ag18769 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
别名 Alpha (1,6) fucosyltransferase, alpha1 6FucT, Fucosyltransferase 8, FUT8
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC025385
基因名称 FUT8
Gene ID (NCBI) 2530
RRIDAB_2881517
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYC5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).