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  • Featured Product
  • KD/KO Validated

FXR1 Monoclonal antibody, PBS Only

FXR1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E12E8

Cat no : 67813-1-PBS

Print datasheet

Synonyms

RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8

验证数据展示

  • WB analysis using 67813-1-Ig (same clone as 67813-1-PBS)

    WB analysis using 67813-1-Ig (same clone as 67813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67813-1-Ig (FXR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    IHC staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67813-1-Ig (FXR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FXR1 antibody included
  • IF Staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    IF Staining of mouse brain using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 67813-1-Ig (same clone as 67813-1-PBS)

    IP experiment of K-562 using 67813-1-Ig (same clone as 67813-1-PBS)

    IP result of anti-FXR1 (IP:67813-1-Ig, 5ug; Detection:67813-1-Ig 1:1000) with K-562 cells lysate 3680 ug. This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    IF Staining of HeLa using 67813-1-Ig (same clone as 67813-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FXR1 antibody (67813-1-Ig, Clone: 1E12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67813-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FXR1 Ready-To-Use IHC Kit. FXR1 primary antibody included.

产品信息

67813-1-PBS targets FXR1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FXR1 fusion protein Ag27800 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fragile X mental retardation, autosomal homolog 1
Synonyms RNA-binding protein FXR1, hFXR1p, FMR1 autosomal homolog 1, 1E12E8
Calculated Molecular Weight 621 aa, 70 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC028983
Gene Symbol FXR1
Gene ID (NCBI) 8087
RRIDAB_2918576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51114
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.