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FXYD3 Polyclonal antibody

FXYD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15853-1-AP

Print datasheet

Synonyms

FXYD3, Mammary tumor 8 kDa protein, MAT 8, MAT8, Phospholemman like, PLML

验证数据展示

  • WB analysis of COLO 320 using 15853-1-AP

    WB analysis of COLO 320 using 15853-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15853-1-AP (FXYD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15853-1-AP

    IHC staining of human breast cancer using 15853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 15853-1-AP

    IHC staining of human breast cancer using 15853-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15853-1-AP (FXYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of COLO 320 using 15853-1-AP

    IP experiment of COLO 320 using 15853-1-AP

    IP result of anti-FXYD3 (IP:15853-1-AP, 4ug; Detection:15853-1-AP 1:500) with COLO 320 cells lysate 2400ug.

经过测试的应用

Positive WB detected inCOLO 320 cells, A375 cells, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15853-1-AP targets FXYD3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FXYD3 fusion protein Ag8672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FXYD domain containing ion transport regulator 3
Synonyms FXYD3, Mammary tumor 8 kDa protein, MAT 8, MAT8, Phospholemman like, PLML
Calculated Molecular Weight 87 aa, 9 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC005238
Gene Symbol FXYD3
Gene ID (NCBI) 5349
RRIDAB_2108602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FXYD3 antibody 15853-1-APDownload protocol
IHC protocol for FXYD3 antibody 15853-1-APDownload protocol
IP protocol for FXYD3 antibody 15853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols