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FYCO1 Polyclonal antibody

FYCO1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25730-1-AP

Print datasheet

Synonyms

FYCO1, RUFY3, ZFYVE7

验证数据展示

  • WB analysis using 25730-1-AP

    WB analysis using 25730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25730-1-AP (FYCO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25730-1-AP

    WB analysis of HeLa using 25730-1-AP

    WB result of FYCO1 antibody (25730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYCO1 transfected HeLa cells.

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, LNCaP cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Immunogen FYCO1 fusion protein Ag22803 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FYVE and coiled-coil domain containing 1
Synonyms FYCO1, RUFY3, ZFYVE7
Calculated Molecular Weight 1478 aa, 167 kDa
Observed Molecular Weight167-180 kDa
GenBank Accession NumberBC007218
Gene Symbol FYCO1
Gene ID (NCBI) 79443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

实验方案

Product Specific Protocols
WB protocol for FYCO1 antibody 25730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF, WB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
View Article