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FZR1/Cdh1 Polyclonal antibody
FZR1/Cdh1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HMy2.CIR cells, C6 cells, HepG2 cells, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, HeLa cells, Jurkat cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16368-1-AP targets FZR1/Cdh1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | FZR1/Cdh1 fusion protein Ag9201 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | fizzy/cell division cycle 20 related 1 (Drosophila) |
Synonyms | FZR1, Fizzy related protein homolog, Cdh1/Hct1 homolog, CDH1, CDC20-like protein 1 |
Calculated Molecular Weight | 493 aa, 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC013413 |
Gene Symbol | FZR1 |
Gene ID (NCBI) | 51343 |
RRID | AB_2107380 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UM11 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IHC protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IF protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
IP protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
FC protocol for FZR1/Cdh1 antibody 16368-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation | ||
Sci Adv The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes.
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Acta Pharmacol Sin Bergenin, a PPARγ agonist, inhibits Th17 differentiation and subsequent neutrophilic asthma by preventing GLS1-dependent glutaminolysis. | ||
Mol Cell Biol ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. | ||
iScience Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.
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