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验证数据展示

  • WB analysis using 32051-1-AP

    WB analysis using 32051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32051-1-AP (Factor IX/PTC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 32051-1-AP

    IHC staining of mouse liver using 32051-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32051-1-AP (Factor IX/PTC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Factor IX/PTC Polyclonal antibody

Factor IX/PTC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32051-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, L02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32051-1-AP targets Factor IX/PTC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Factor IX/PTC fusion protein Eg1045 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor IX
别名 F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain
计算分子量52kDa
观测分子量30-33 kDa, 50 kDa
GenBank蛋白编号NM_000133.4
基因名称 F9
Gene ID (NCBI) 2158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP00740-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond.

实验方案

Product Specific Protocols
WB protocol for Factor IX/PTC antibody 32051-1-APDownload protocol
IHC protocol for Factor IX/PTC antibody 32051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols