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验证数据展示

  • WB analysis of human plasma using 66753-1-Ig (same clone as 66753-1-PBS)

    WB analysis of human plasma using 66753-1-Ig (same clone as 66753-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66753-1-Ig (Factor X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    IHC staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66753-1-Ig (Factor X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    IF Staining of human liver using 66753-1-Ig (same clone as 66753-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Factor X antibody (66753-1-Ig, Clone: 3D3F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66753-1-PBS in a different storage buffer formulation.

Factor X Monoclonal antibody, PBS Only

Factor X Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66753-1-PBS

产品说明书

CloneNo. 3D3F11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

F10, Factor X heavy chain, EC:3.4.21.6, coagulation factor X, Activated factor Xa heavy chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66753-1-PBS targets Factor X in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 Factor X fusion protein Ag11664 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coagulation factor X
别名 F10, Factor X heavy chain, EC:3.4.21.6, coagulation factor X, Activated factor Xa heavy chain
计算分子量 488 aa, 55 kDa
观测分子量 50 kDa
GenBank蛋白编号BC046125
基因名称 F10
Gene ID (NCBI) 2159
RRIDAB_2882099
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00742
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.