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Species Reactivity
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验证数据展示

  • WB analysis using 30601-1-AP

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30601-1-AP

    WB analysis using 30601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 30601-1-AP

    IF Staining of Raji using 30601-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Fas/CD95 Polyclonal antibody

Fas/CD95 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30601-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Fas, CD95, TNFR6, APT1, APO 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, J774A.1 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30601-1-AP targets Fas/CD95 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Fas/CD95 fusion protein Ag30426 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fas (TNF receptor superfamily member 6)
别名 Fas, CD95, TNFR6, APT1, APO 1
计算分子量 37 kDa
观测分子量 30-40 kDa
GenBank蛋白编号NM_007987
基因名称 Fas/CD95
Gene ID (NCBI) 14102
RRIDAB_3086371
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25446
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

实验方案

Product Specific Protocols
WB protocol for Fas/CD95 antibody 30601-1-APDownload protocol
IF protocol for Fas/CD95 antibody 30601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols