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Anti-Human Fas/CD95 Rabbit Recombinant Antibody

Fas/CD95 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240340G11

Cat no : 98034-2-RR

Print datasheet

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

验证数据展示

  • FC experiment of human PBMCs using 98034-2-RR

    FC experiment of human PBMCs using 98034-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD45RO (UCHL1) (CL647-65150, Clone: UCHL1). Cells were not fixed.

  • FC experiment of human PBMCs using 98034-2-RR

    FC experiment of human PBMCs using 98034-2-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-2-RR, Clone: 240340G11) (red) or Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98034-2-RR

    Affinity and Kinetic Characterization of 98034-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98034-2-RR against Human Fas/CD95 were performed. The affinity constant is 0.191 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98034-2-RR targets Fas/CD95 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fas (TNF receptor superfamily, member 6)
Synonyms FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1
Calculated Molecular Weight38 kDa
GenBank Accession NumberNM_000043.6
Gene Symbol Fas/CD95
Gene ID (NCBI) 355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25445-1
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

实验方案

Product Specific Protocols
FC protocol for Fas/CD95 antibody 98034-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols