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验证数据展示

  • IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Fascin antibody included
  • IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Fascin antibody included
  • IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66321-1-Ig (Fascin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66321-1-Ig(Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Fascin antibody included
  • IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody (66321-1-Ig, Clone: 1B5G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Fascin Monoclonal antibody, PBS Only

Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66321-1-PBS

产品说明书

CloneNo. 1B5G3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.

产品信息

66321-1-PBS targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Fascin fusion protein Ag6074 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
别名 FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC000521
基因名称 Fascin
Gene ID (NCBI) 6624
ENSEMBL Gene IDENSG00000075618
RRIDAB_2881701
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16658
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.