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  • Featured Product
  • KD/KO Validated

Fascin Monoclonal antibody, PBS Only

Fascin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5G3

Cat no : 66321-1-PBS

Print datasheet

Synonyms

FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein

验证数据展示

  • IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Fascin antibody included
  • IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IHC staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66321-1-Ig (Fascin Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Fascin antibody included
  • IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66321-1-Ig (Fascin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    WB analysis of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66321-1-Ig (Fascin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66321-1-Ig(Fascin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    FC experiment of SH-SY5Y using 66321-1-Ig (same clone as 66321-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Fascin Monoclonal antibody (66321-1-Ig, Clone:1B5G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    IF Staining of human tonsillitis using 66321-1-Ig (same clone as 66321-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Fascin antibody (66321-1-Ig, Clone: 1B5G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66321-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Fascin Ready-To-Use IHC Kit. Fascin primary antibody included.

产品信息

66321-1-PBS targets Fascin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fascin fusion protein Ag6074 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Synonyms FSCN1, Fascin 1,Fascin, FAN1, 55 kDa actin-bundling protein, 55 kDa actin bundling protein
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000521
Gene Symbol Fascin
Gene ID (NCBI) 6624
ENSEMBL Gene IDENSG00000075618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16658
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fascin is a highly conserved and ubiquitous actin cross-linking protein that has a major function in cell motility and adhesion. Fascin localizes to a number of highly dynamic cellular structures that require strong mechanical support which include stress fibers and cellular protrusions such as microvilli, microspikes and lamellipodia. Fascin is up-regulated in many human carcinomas. It's a marker for Reed-Sternberg cells in Hodgkin's disease; it is also a highly selective marker for dendritic cells of lymphoid tissues.