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Ferrostatin-1

Ferrostatin-1 (Fer-1) 是一种选择性铁死亡抑制剂，有效抑制 Erastin 诱导的 HT-1080 细胞铁死亡，EC50值为60 nM。Ferrostatin-1有抗氧化和抗真菌活性。

CAS号

347174-05-4

分子式

C15H22N2O2

主要靶点

Antifungal|Ferroptosis

仅限科研使用

Cat No : CM00719

Print datasheet

Synonyms

Ferrostatin 1|Ferrostatin-1 (Fer-1)

验证数据展示

  • CAS No.: 347174-05-4

    Ferrostatin-1
    CAS#: 347174-05-4

产品信息

Ferrostatin-1 is a potent ferroptosis inhibitor (EC50: 60 nM).

CAS号 347174-05-4
分子式 C15H22N2O2
主要靶点 Antifungal|Ferroptosis
主要通路 微生物学|凋亡
分子量 262.35
纯度 99.51%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 Ferrostatin 1|Ferrostatin-1 (Fer-1)

靶点活性

ferroptosis:60 nM(EC50, HT-1080 cells)

体外活性

Ferrostatin-1 (Fer-1) is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 did prevent erastin-induced accumulation of cytosolic and lipid ROS [1]. Ferrostatin-1 inhibited cell death in cellular models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction; Fer-1 inhibited lipid peroxidation, but not mitochondrial reactive oxygen species formation or lysosomal membrane permeability [2]. Fer-1 is not toxic toward the SH-SY5Y cells at concentrations up to 12.5 μM. Fer-1 compound quenched the commercially available stable radical DPPH, in non-cellular assay at 82 %. Fer-1 inhibited the ROS/RNS generated under rotenone insult in SH-SY5Y cells [3].

溶解度

Ethanol:26.2 mg/mL (100 mM),DMSO:200 mg/mL (762.3 mM)

细胞实验

Cell viability was typically assessed in 384-well format by Alamar Blue fluorescence (ex/em 530/590) measured on a Victor3 plate reader. In some experiments, Trypan Blue dye exclusion counting was performed using an automated cell counter. Cell viability under test conditions is reported as a percentage relative to the negative control treatment [1].

参考文献

1.Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.
2.Skouta R, et al. Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models. J Am Chem Soc. 2014 Mar 26;136(12):4551-6.
3.Kabiraj P, et al. The neuroprotective role of ferrostatin-1 under rotenone-induced oxidative stress in dopaminergic neuroblastoma cells. Protein J. 2015 Oct;34(5):349-58.
4.Su G, Yang W, Wang S, et al. SIRT1-autophagy axis inhibits excess iron-induced ferroptosis of foam cells and subsequently increases IL-1Β and IL-18[J]. Biochemical and Biophysical Research Communications. 2021, 561: 33-39.
5.Sun Y, He L, Wang T, et al. Activation of p62-Keap1-Nrf2 Pathway Protects 6-Hydroxydopamine-Induced Ferroptosis in Dopaminergic Cells[J]. Molecular Neurobiology. 2020, 57(11): 4628-4641.
6.Chen X, Gao C, Cheng Z, et al. Ruxolitinib exerts neuroprotection via repressing ferroptosis in a mouse model of traumatic brain injury[J]. Experimental Neurology. 2021: 113762.
7.Yin W, Luan X, Li Z, et al. Structural basis for inhibition of the SARS-CoV-2 RNA polymerase by suramin[J]. Nature Structural & Molecular Biology. 2021: 1-7.
8.Wang S, Li F, Qiao R, et al. Arginine-Rich Manganese Silicate Nanobubbles as a Ferroptosis-Inducing Agent for Tumor-Targeted Theranostics[J]. ACS nano. 2018 Dec 26;12(12):12380-12392.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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