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验证数据展示

  • WB analysis of human heart using 51015-1-AP

    WB analysis of human heart using 51015-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 51015-1-AP (Fhl1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 51015-1-AP

    IHC staining of human ovary tumor using 51015-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51015-1-AP (Fhl1 antibody) at dilution of 1:50 (under 10x lens).

Fhl1 Polyclonal antibody

Fhl1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51015-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Fhl1, four and a half LIM domains 1, KyoT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51015-1-AP targets Fhl1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 four and a half LIM domains 1
别名 Fhl1, four and a half LIM domains 1, KyoT
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC031120
基因名称 Fhl1
Gene ID (NCBI) 14199
RRIDAB_615005
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP97447
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Fhl1 antibody 51015-1-APDownload protocol
IHC protocol for Fhl1 antibody 51015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols