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Fibrillin 1 Polyclonal antibody

Fibrillin 1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26935-1-AP

Print datasheet

Synonyms

FBN, FBN1, fibrillin 1, MASS, MFS1, OCTD, SGS, WMS

验证数据展示

  • IHC staining of human breast cancer using 26935-1-AP

    IHC staining of human breast cancer using 26935-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26935-1-AP (Fibrillin 1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26935-1-AP

    IHC staining of human breast cancer using 26935-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26935-1-AP (Fibrillin 1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 26935-1-AP

    IF Staining of SH-SY5Y using 26935-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 26935-1-AP (Fibrillin 1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

26935-1-AP targets Fibrillin 1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Fibrillin 1 fusion protein Ag25342 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibrillin 1
Synonyms FBN, FBN1, fibrillin 1, MASS, MFS1, OCTD, SGS, WMS
GenBank Accession NumberBC146854
Gene Symbol Fibrillin 1
Gene ID (NCBI) 2200
RRIDAB_2880690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35555
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillin-1 belongs to the fibrillin family. Fibrillins are structural components of 10-12 nm extracellular calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1-containing microfibrils provide long-term force bearing structural support. Defects in Fibrillin-1 are a cause of Marfan syndrome (MFS), isolated ectopia lentis (EL), Weill-Marchesani syndrome autosomal dominant (ADWMS), Shprintzen-Goldberg craniosynostosis syndrome (SGS) or MASS syndrome. This antibody is specific to Fibrillin-1.

实验方案

Product Specific Protocols
IHC protocol for Fibrillin 1 antibody 26935-1-APDownload protocol
IF protocol for Fibrillin 1 antibody 26935-1-APDownload protocol
FC protocol for Fibrillin 1 antibody 26935-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Deciphering the immune landscape dominated by cancer-associated fibroblasts to investigate their potential in indicating prognosis and guiding therapeutic regimens in high grade serous ovarian carcinoma

Authors - Yimin Li
View Article