Fibrillin 1 Polyclonal antibody

Fibrillin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29425-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

FBN1, Asprosin, FBN, Fibrillin-1, MASS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29425-1-AP targets Fibrillin 1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性mouse
免疫原 Fibrillin 1 fusion protein Ag30283 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibrillin 1
别名 FBN1, Asprosin, FBN, Fibrillin-1, MASS
观测分子量350 kDa
GenBank蛋白编号BC146854
基因名称 Fibrillin 1
Gene ID (NCBI) 2200
RRIDAB_3086131
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibrillin 1 is a 350 kDa large extracellular matrix glycoprotein that formed thread-like microfibril filaments, which provided structural support for tissues, formed elastic fibers in the skin and vessels, regulated the availability of the transforming growth factor beta (TGF-β) protein, and modulated the microenvironment. Fibrillin 1 contains a large number of cysteine-rich epidermal growth factor (EGF)- like domains, most capable of binding calcium ions (cbEGF-like), and a few motifs homologous to the binding protein for transforming growth factor beta (TGFβ1-BP)- like module (PMID: 29220879, PMID: 35234370).

实验方案

Product Specific Protocols
WB protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IHC protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
IF protocol for Fibrillin 1 antibody 29425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Cells

ADAR1 Is Essential for Smooth Muscle Homeostasis and Vascular Integrity

Authors - Dunpeng Cai
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