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验证数据展示

  • IHC staining of human breast cancer using 27596-1-AP

    IHC staining of human breast cancer using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAP antibody included
  • IHC staining of human breast cancer using 27596-1-AP

    IHC staining of human breast cancer using 27596-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27596-1-AP (Fibroblast activation protein alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAP antibody included

FAP Polyclonal antibody

FAP Polyclonal Antibody for IHC, ELISA
Cat No. 27596-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

FAPA, 170 kDa melanoma membrane-bound gelatinase, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP, EC:3.4.14.5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAP Ready-To-Use IHC Kit. FAP primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27596-1-AP targets FAP in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 FAP fusion protein Ag26695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast activation protein, alpha
别名 FAPA, 170 kDa melanoma membrane-bound gelatinase, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP, EC:3.4.14.5
计算分子量 760 aa, 88 kDa
GenBank蛋白编号BC026250
基因名称 FAP
Gene ID (NCBI) 2191
RRIDAB_3085973
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for FAP antibody 27596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols