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  • Featured Product
  • KD/KO Validated

Fibulin 5 Monoclonal antibody, PBS Only

Fibulin 5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5C8

Cat no : 60081-1-PBS

Print datasheet

Synonyms

ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein

验证数据展示

  • WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60081-1-Ig (Fibulin 5 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB analysis of MCF-7 using 60081-1-Ig (same clone as 60081-1-PBS)

    WB result of Fibulin 5 antibody (60081-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Fibulin 5 transfected MCF-7 cells. This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

    IF Staining of A549 using 60081-1-Ig (same clone as 60081-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed A549 cells using 60081-1-Ig(Fibulin 5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60081-1-PBS in a different storage buffer formulation.

产品信息

60081-1-PBS targets Fibulin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Fibulin 5 fusion protein Ag2859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fibulin 5
Synonyms ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, FLJ90059, UP50, Urine p50 protein
Calculated Molecular Weight 448 aa, 50 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC022280
Gene Symbol Fibulin 5
Gene ID (NCBI) 10516
RRIDAB_2231920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UBX5
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.