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Flightless I Monoclonal antibody, PBS Only

Flightless I Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9C8

Cat no : 67039-1-PBS

Print datasheet

Synonyms

FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog

验证数据展示

  • WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

    WB analysis using 67039-1-Ig (same clone as 67039-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67039-1-Ig (FLII antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67039-1-Ig (same clone as 67039-1-PBS)

    IHC staining of human colon cancer using 67039-1-Ig (same clone as 67039-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67039-1-Ig (FLII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67039-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Flightless I antibody included
Planning an IHC experiment? We recommend our IHCeasy Flightless I Ready-To-Use IHC Kit. Flightless I primary antibody included.

产品信息

67039-1-PBS targets Flightless I in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Flightless I fusion protein Ag26865 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name flightless I homolog (Drosophila)
Synonyms FLI, Fli1, Flightless I, FLII, FLIL, Protein flightless 1 homolog
Calculated Molecular Weight 1269 aa, 145 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC025300
Gene Symbol Flightless I
Gene ID (NCBI) 2314
RRIDAB_2882353
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13045
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).