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Species Reactivity
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验证数据展示

  • WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

    WB analysis using 83598-4-RR (same clone as 83598-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83598-4-RR (Flotillin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

    IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included
  • IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

    IHC staining of human stomach cancer using 83598-4-RR (same clone as 83598-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83598-4-RR (Flotillin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT1 antibody included
  • IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

    IP experiment of mouse brain using 83598-4-RR (same clone as 83598-4-PBS)

    IP result of anti-Flotillin 1 (IP:83598-4-RR, 4ug; Detection:83598-4-RR 1:3000) with mouse brain tissue lysate 2400 ug. This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83598-4-RR (same clone as 83598-4-PBS)

    IF Staining of HeLa using 83598-4-RR (same clone as 83598-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Flotillin 1 antibody (83598-4-RR, Clone: 240582G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83598-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83598-4-RR

    Affinity and Kinetic Characterization of 83598-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83598-4-RR against Human Flotillin 1 were performed. The affinity constant is 30.5 nM.

Flotillin 1 Recombinant antibody, PBS Only

Flotillin 1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 83598-4-PBS

产品说明书

CloneNo. 240582G6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

FLOT1, 240582G6, Flotillin-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy FLOT1 Ready-To-Use IHC Kit. FLOT1 primary antibody included.

产品信息

83598-4-PBS targets Flotillin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Flotillin 1 fusion protein Ag7902 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 flotillin 1
别名 FLOT1, 240582G6, Flotillin-1
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC001146
基因名称 Flotillin 1
Gene ID (NCBI) 10211
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO75955
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.